FACULTY
OF MEDICAL SCIENCE
VIROLOGY INSTITUTE
VIRUS-CELL INTERACTIONS
LABORATORY
RESEARCH AND TEACHING
Dr. Severo Paglini – Consult Professor in Activity
Dr. María Gabriela Paglini – Chief
of Practical Work – Member of the Research Career
of the National Research Council of the
Contact Addres:
Dr. Severo Paglini – Instituto de Virología
Ciudad Universitaria – 5000 Córdoba – R. Argentina – E-mail: spaglini@fcm.unc.edu.ar -
spaglini@cmefcm.uncor.edu
June 16, 2007
This web site
has been developed to inform, receive and exchange experiences related to virus
and cellular receptors to which the viruses bind as a preliminary step to get
inside the cell. Also, we are studying the virus-cell interactions, especially
with the cytoplasm components like the cytoskeleton, mitochondrias and
endosomes.
The virus model used in our lab is Pixuna, Togaviridae family, alfa genus, Venezuelan complex, that has the advantage that
does not produce illness in human being.
We
have isolated and studied a receptor present in the brain of 10 days-old Swiss
albino mice. The presence of gangliosides could be determined, which have the
ability to inhibit the hemagglutination of Pixuna virus as well as that of
other members of the genus. We have been able to prove that the sialic acid is
the active group and to demonstrate that that viruses bind to 2-3 linked sialic
acid present in the GM1 ganglioside.
Another receptor studied is the
present in erythrocytes of 1 day-old
chicken (Paglini, S. Pereyra, E. del C.:
Caracterización parcial de un receptor para el virus Pixuna present
en eritrocitos de pollos de 1 día de edad. Rev.
The
Lab has studied the receptor present in 10 days old chicken embryo fibroblasts.
With the purified receptor poly and monoclonal antibodies has been prepared and
Inmunofluorescence studies were performed. Electron microscopic studies
together with the inmunofluorescence has showed that
the receptor is a membranous structure. The Western blot test has demonstrated
2 proteic bands of around 60 kD that correspond to the receptor. This study has
been recently published: Paglini, MG, Pereyra, E del
C. and Paglini, S. Cellular receptor for Pixuna virus in chicken embryonic
fibroblasts. Med. Microbiol. Immunol. 195, 85-92 2006.
The
Lab is studying the mitochondrias, the endosomes and the cytoskeleton
participation in the Pixuna virus replication. Using Mito Tracker and electron
microscopy we were able to show the mitochondrias participation in the nucleocapside
formation. The drug glucosamina was able to modify the pH of the endosomes. At
the moment we are experiencing the drug Bafilomycin
A1 to study the proton pump in the Pixuna virus replication.
A
recent book:
VIRUS RECEPTORES Y CORRECEPTORES CELULARES.
Severo Paglini with the collaboration of: Aldo R. Eynard y Reyna O. Calderón.
Editorial: CTM, Servicios Bibliográficos S.A.
Junín 925 - 1133 Ciudad de Buenos Aires - R. Argentina.